About FL114: 

FL114 is an innovative, privately held biotechnology company developing our novel epigenetic editing platform to treat a variety of diseases through precise, tunable modification of gene expression. FL114 was founded by Flagship Pioneering, where companies such as Moderna Therapeutics (NASDAQ: MRNA) and Seres Therapeutics (NASDAQ: MCRB) were conceived and created.  With a strong emphasis on innovation and experimental execution, FL114 cultivates a highly dynamic and entrepreneurial environment.  

About the Role:

FL114, Inc. is seeking a highly motivated and detail-oriented Senior Research Associate / Associate Scientist to join our Therapeutics Engineering team. The successful candidate will play a critical role in the design and execution of high-throughput screening (HTS) campaigns, maintaining cell culture, and performing molecular biology techniques to support early-stage drug discovery efforts. This is a hands-on laboratory-based position offering the opportunity to work in a dynamic, team-oriented environment at the forefront of therapeutic innovation.

Key Responsibilities:

• Perform cell-based and biochemical high-throughput screening assays to identify and characterize epigenetic regulators.
• Culture and maintain a wide variety of mammalian cell lines, including transient and stable transfections – (Lentiviral work – packaging virus, titering virus, transducing cell lines with selection).
• Execute routine and advanced molecular biology techniques, including plasmid preparation, RT-qPCR, cloning, RNA/DNA extraction, gel electrophoresis, oligo pool work, etc.
• Analyze and interpret assay data, maintain accurate laboratory notebooks, and communicate results clearly in team meetings.
• Assist in the optimization and automation of screening protocols, including use of liquid handling systems and plate readers.
• Support assay development, validation, and troubleshooting efforts in collaboration with other team members.
• Ensure adherence to laboratory safety practices and regulatory guidelines.

Required Skills / Qualifications:

• Bachelor’s or Master’s degree in Cell Biology, Molecular Biology, Biochemistry, or a related field.
• Strong hands-on experience with cell culture (adherent and suspension), including aseptic technique and routine maintenance of immortalized and primary cells.
• Proficiency in molecular biology techniques such as cloning, PCR, and nucleic acid extraction.
• Familiarity with HTS platforms, plate-based assay design, and use of automation tools and software.
• Excellent organizational, communication, and team collaboration skills.
• Comfortable working in a fast-paced, multitasking environment.
• Prefer experience with CRISPR editing or viral vector production; CRISPR screening
• Prefer familiarity with data analysis tools such as GraphPad Prism, Benchling or SnapGene.
• Prefer prior experience working in drug discovery or pharmaceutical R&D.